Streptomyces paludis sp. nov., isolated from an alpine wetland soil

A Gram-stain-positive actinobacterium, designated strain GSSD-12^T, was isolated from an alpine wetland soil. blast search of the full-length 16S rRNA gene sequence of GSSD-12^T indicated it represented a member of the genus Streptomyces, and displayed highest similarity with Streptomyces scopuliridis NRRL B-24574^T (99.2 %) and less than 98.6 % similarity with other species of the genus Streptomyces with validly published names. Phylogenetic analysis based on 16S rRNA gene sequences indicated that GSSD-12^T was closely related to S. scopuliridis NRRL B-24574^T, Streptomyces odonnellii NRRL B-24891^T and Streptomyces lushanensis NRRL B-24994^T. However, the digital DNA-DNA hybridization value, the average nucleotide identity value and the multilocus sequence analysis evolutionary distance between this strain and its closest relatives indicated that it represented a distinct species. Furthermore, GSSD-12^T was also distinctly differentiated from them by morphological, physiological and biochemical characteristics. Therefore, strain GSSD-12^T(=CICC 11051^T=JCM 33019^T) represents a novel species of the genus Streptomyces, for which the name Streptomyces paludis sp. nov. is proposed.