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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jan 4, 2026

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Published on: October 4, 2018

Phosphoflow cytometry and barcoding in blood platelets: Technical and analytical considerations.

Benjamin E J Spurgeon¹, Khalid M Naseem²

¹Center for Platelet Research Studies, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Harvard Medical School, Boston, Massachusetts.

Cytometry. Part B, Clinical Cytometry

|November 2, 2019

Summary

Multiplex flow cytometry enables rapid analysis of platelet phosphorylation signals, crucial for understanding cardiovascular events and developing new antiplatelet therapies. Standardizing these assays is key for clinical application.

Keywords:

blood platelets cardiovascular diseases flow cytometry phosphorylation platelet function tests

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Last Updated: Jan 4, 2026

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Published on: November 29, 2024

Area of Science:

Biochemistry
Immunology
Cardiovascular Science

Background:

Platelet function relies on precise phosphoprotein signaling, with aberrations linked to cardiovascular events.
Current assays for platelet phosphorylation are low-throughput and not clinically viable.
Multiplex flow cytometry offers a high-throughput alternative for analyzing platelet signaling.

Purpose of the Study:

To review key technical steps for successful multiplex flow cytometry assays of platelet phosphorylation.
To guide standardization efforts for clinical application of platelet function assays.

Main Methods:

Review of technical protocols for multiplex flow cytometry.
Emphasis on critical steps: fixation, permeabilization, staining, controls, and data visualization.

Main Results:

Multiplex flow cytometry allows rapid, high-throughput acquisition of platelet phosphorylation data.
Standardization of protocols and reagents is essential for assay reproducibility.
Detailed review of technical considerations for successful implementation.

Conclusions:

Standardized multiplex flow cytometry can accelerate the discovery of novel antiplatelet agents.
Improved platelet function analysis enhances cardiovascular disease management.
This review provides a framework for optimizing platelet phosphorylation assays.