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SPARCL1 impedes trophoblast migration and invasion by down-regulating ERK phosphorylation and AP-1 production and

Xiaojing Liu1, Jun Zhao1, Xiaorui Luan1

  • 1Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200135, China; Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, 200135, China.

Placenta
|November 2, 2019
PubMed
Abstract

Insights

Secreted protein acidic and rich in cysteine like-1 (SPARCL1) was elevated in spontaneous abortion cases. Overexpression of SPARCL1 inhibited trophoblast cell migration and invasion, suggesting a role in pregnancy complications.

Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Molecular Biology

Background:

  • Embryo implantation relies on trophoblast cell migration and invasion.
  • Dysfunctional trophoblast cells are linked to pregnancy complications.
  • Secreted protein acidic and rich in cysteine like-1 (SPARCL1) inhibits cell migration and tumor invasion.

Purpose of the Study:

  • To investigate the role of SPARCL1 in human trophoblast cell function.
  • To determine SPARCL1 expression in spontaneous abortion.
  • To explore SPARCL1's impact on trophoblast cell migration, invasion, and related molecular pathways.

Main Methods:

  • Analysis of SPARCL1 expression in villous specimens from normal and spontaneous abortion pregnancies.
  • Transfection of trophoblast cell lines (HTR8/SVneo, JAR) to overexpress SPARCL1.
  • Assays for cell proliferation, migration (scratch-wound), epithelial-mesenchymal transition (EMT) markers, ERK phosphorylation, and AP-1 expression.

Main Results:

  • SPARCL1 mRNA and protein levels were significantly higher in the spontaneous abortion group.
  • SPARCL1 overexpression did not affect trophoblast cell viability but reduced migration distance and area.
  • SPARCL1 overexpression altered EMT markers and decreased ERK phosphorylation and AP-1 (Fos, Jun) expression.

Conclusions:

  • Overexpression of SPARCL1 restrains trophoblast cell migration and invasion.
  • SPARCL1 may negatively impact embryo implantation and placenta development.
  • Elevated SPARCL1 could be implicated in the pathogenesis of spontaneous abortion.
Keywords:
EMTImplantationSPARCL1Spontaneous abortionTrophoblast migration

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