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Metabolism of glutamate and ammonia in astrocyte: an immunocytochemical study
T Kaneko1, R Shigemoto, N Mizuno
1Department of Anatomy (1st Division), Faculty of Medicine, Kyoto University, Japan.
Brain Research
|August 2, 1988
Abstract:
Alpha-ketoglutarate (alpha-KG) reductive amination activity in rat brain was found to be mostly absorbed with an antibody against liver glutamate dehydrogenase. With this and anti-glutamine synthetase antibodies, alpha-KG reductive amination activity was immunocytochemically shown to coexist with glutamine synthetase activity in astrocytes. The results suggest that astrocytes de novo synthesize glutamate from alpha-KG and ammonia, and metabolize it to glutamine.