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BBC microcomputer controlled field inversion gel electrophoresis.

C D Porter¹, A G Porter, L C Archard

¹Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK.

Computer Applications in the Biosciences : CABIOS

|April 1, 1988

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This study presents a low-cost, homemade control unit for field inversion gel electrophoresis, enabling better separation of large DNA molecules using conventional equipment. This method overcomes the size limitations of standard gel electrophoresis without expensive pulsed-field systems.

Area of Science:

Molecular Biology
Biotechnology
Biophysics

Background:

Agarose gel electrophoresis is a standard technique for DNA separation.
Conventional methods have limitations in resolving large DNA fragments.
Pulsed-field gel electrophoresis extends resolution but requires costly apparatus.

Purpose of the Study:

To develop an affordable system for enhanced DNA size separation.
To adapt existing electrophoresis equipment for field inversion gel electrophoresis.
To overcome the size limitations of standard gel electrophoresis.

Main Methods:

Construction of a custom control unit.
Interfacing the control unit with a BBC microcomputer.
Utilizing field inversion gel electrophoresis principles.

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Main Results:

Successful implementation of a home-made control unit.
Demonstrated capability for field inversion gel electrophoresis.
Extended resolution of large DNA molecules.

Conclusions:

The developed system provides a cost-effective alternative for large DNA molecule separation.
This approach democratizes advanced gel electrophoresis techniques.
It enables researchers to overcome size limitations without significant capital investment.