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Estimation of Cell Number by Hemocytometry Counting.

Michael R Green, Joseph Sambrook

    Cold Spring Harbor Protocols
    |November 3, 2019
    PubMed
    Summary

    Counting mammalian cells in a specific volume is crucial. Hemocytometers offer a method, but automated cell-counting devices provide efficiency for numerous samples.

    Area of Science:

    • Cell biology
    • Biotechnology

    Background:

    • Accurate cell enumeration is vital for various biological and medical applications.
    • Traditional methods like hemocytometers are manual and can be time-consuming.
    • Automated cell counters offer a more efficient alternative for high-throughput sample analysis.

    Purpose of the Study:

    • To describe the principles and methodology of using a hemocytometer for mammalian cell counting.
    • To highlight the advantages of automated cell-counting devices for large-scale sample analysis.

    Main Methods:

    • Utilizing a hemocytometer with two chambers, each holding 9 µL.
    • Employing a grid system within each chamber, with each square measuring 1 × 1 mm and a depth of 0.1 mm.
    • Recognizing that each square's volume is 0.1 mm³ (0.1 µL) when coverslipped, with further subdivisions being unnecessary for counting.

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    Main Results:

    • The hemocytometer's geometric design allows for precise volume determination of sampled medium.
    • Each square within the hemocytometer chamber represents a defined volume (0.1 µL) for cell counting.
    • Automated cell counters are presented as a more efficient alternative for processing numerous samples.

    Conclusions:

    • Hemocytometers provide a foundational method for determining mammalian cell concentration in a defined volume.
    • Automated cell-counting technologies are recommended for scenarios requiring high-throughput sample processing.
    • Understanding the precise volume of hemocytometer squares is key to accurate manual cell enumeration.