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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
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Preparing Feeder Cell Cultures to Support Hybridoma Growth
Cold Spring Harbor Protocols
|November 3, 2019
Abstract:
Feeder layer plates are prepared with cells that provide the appropriate growth factors to support the growth of hybridoma cells until they can expand in number and provide their own. Good feeder cells should have properties that allow them to be selected against during the future growth of the hybridomas. Peritoneal macrophages, myeloma cells, splenocytes, and MRC-5 cells (a human lung fibroblast line) are the most common feeder layer cells used in hybridoma fusions. Methods for their preparation are described here.
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