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Liposomal delivery of CRISPR/Cas9
Shuai Zhen1,2,3, Xu Li4,5
1Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, 710061, Xi'an, Shaanxi, PR China.
Cancer Gene Therapy
|November 3, 2019
Summary
Liposomes are effective carriers for CRISPR/Cas9 delivery, with surface properties like charge and modification influencing gene silencing. Cationic and neutral liposomes show promise, overcoming systemic delivery barriers.
Area of Science:
- Biotechnology and Nanomedicine
- Gene Editing Technologies
- Drug Delivery Systems
Background:
- Liposomes are extensively studied for CRISPR/Cas9 delivery.
- Surface properties (charge, PEGylation, ligand modification) critically impact gene silencing efficiency.
- Systemic delivery faces barriers: circulation time, tumor penetration, and cellular uptake.
Purpose of the Study:
- Analyze how liposomal surface properties affect CRISPR/Cas9 delivery barriers.
- Evaluate the performance of liposomes with varying surface characteristics.
- Discuss challenges and future directions in liposomal CRISPR/Cas9 systems.
Main Methods:
- Investigated liposomal carriers with diverse surface charges (cationic, neutral, anionic).
- Examined the effects of PEGylation and ligand modification on liposome performance.
- Assessed liposome efficacy in overcoming systemic delivery hurdles.
Main Results:
- Cationic and neutral liposomal formulations demonstrate strong CRISPR/Cas9 delivery capabilities.
- Anionic formulations are generally unsuitable for CRISPR/Cas9 delivery.
- PEGylation presents a dilemma between circulation time and cellular uptake; repeated use causes accelerated blood clearance.
Conclusions:
- Liposomal surface properties are key determinants of CRISPR/Cas9 delivery efficiency.
- Cationic and neutral liposomes are promising for gene editing applications.
- Further research is needed to optimize liposomal formulations and overcome delivery challenges.
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