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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
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Complete genome sequence of aglaonema bacilliform virus (ABV)
R A Alvarez-Quinto1, B E L Lockhart2, J M Moreno-Martinez2
1Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN, 55108, USA. alvar419@umn.edu.
Archives of Virology
|November 4, 2019
Summary
Aglaonema bacilliform virus (ABV) causes leaf issues in Aglaonema plants. Genomic analysis reveals its relation to taro bacilliform CH virus, aiding in understanding plant virus evolution.
Area of Science:
- Plant Virology
- Molecular Biology
- Genomics
Background:
- Aglaonema bacilliform virus (ABV) is a plant pathogen.
- ABV belongs to the genus Badnavirus within the family Caulimoviridae.
- It causes leaf deformation and chlorosis in Aglaonema modestum.
Purpose of the Study:
- To determine the complete genome sequence of an Aglaonema bacilliform virus (ABV) isolate.
- To analyze the genomic organization and protein-coding potential of ABV.
- To understand the phylogenetic relationship of ABV with other badnaviruses.
Main Methods:
- Whole-genome sequencing of the ABV isolate.
- Bioinformatic analysis of open reading frames (ORFs) and protein domains.
- Phylogenetic analysis using amino acid sequences, particularly the reverse transcriptase/RNase H region.
Main Results:
- The complete genome of the Minnesota ABV isolate is 7,178 bp long.
- The genome contains three ORFs encoding proteins of 14.92, 13.33, and 207.95 kDa.
- Phylogenetic analysis places ABV closely with taro bacilliform CH virus (TaBCHV), sharing 71% nucleotide identity in the RT/RNase H region.
Conclusions:
- The genome structure of ABV is typical for the genus Badnavirus.
- ABV is closely related to TaBCHV, suggesting potential shared origins or transmission pathways.
- This study provides genomic insights into ABV, contributing to plant virus research.
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