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Related Concept Videos

Blood Studies for Cardiovascular System I: Cardiac Biomarkers01:20

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

740
Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
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Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers01:19

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

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Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
498

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A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
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Development Toward a Triple-Marker Biosensor for Diagnosing Cardiovascular Disease.

Anna Deng1, Daniel Matloff1, Chi-En Lin1

  • 1School of Biological and Health Systems Engineering, Arizona State University, Tempe, Arizona.

Critical Reviews in Biomedical Engineering
|November 4, 2019
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New biosensors offer rapid, label-free detection of key cardiac biomarkers, including B-type natriuretic peptide (BNP), cardiac troponin I (cTnI), and C-reactive protein (CRP). These advancements aim to simplify cardiovascular disease diagnosis and management.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Cardiovascular Medicine

Background:

  • Cardiovascular disease (CVD) is a leading global cause of mortality, necessitating accurate and efficient diagnostic methods.
  • Current assays for cardiac biomarkers are often complex and time-consuming, hindering timely diagnosis and management.
  • There is a critical need for rapid, sensitive, and user-friendly biosensors for monitoring multiple cardiac biomarkers.

Purpose of the Study:

  • To develop and report rapid, label-free biosensor prototypes for three FDA-approved cardiac biomarkers: BNP, cTnI, and CRP.
  • To demonstrate the capability of these biosensors for sensitive, multi-marker detection using electrochemical impedance spectroscopy (EIS).
  • To evaluate the clinical relevance of the developed biosensors, particularly for BNP detection in blood samples.

Main Methods:

  • Fabrication of biosensors by immobilizing antibodies for BNP, cTnI, and CRP onto gold working electrodes.
  • Utilized electrochemical impedance spectroscopy (EIS) for label-free detection and quantification of biomarkers.
  • Determined the optimal frequency (OF) for each biomarker to enable sensitive and specific detection.

Main Results:

  • Successfully identified optimal frequencies (OFs) for BNP (1.74 Hz), cTnI (37.56 Hz), and CRP (253.9 Hz).
  • Demonstrated femto-molar sensitivity using EIS, enabling label-free detection of multiple biomarkers.
  • The BNP biosensor achieved a clinically relevant detection limit of 100 pg/mL when tested in blood samples.

Conclusions:

  • Developed rapid, label-free biosensor prototypes for key cardiac biomarkers (BNP, cTnI, CRP).
  • EIS proves to be a sensitive technique for multi-marker detection when optimal frequencies are identified.
  • These biosensors hold significant potential for simplifying and improving cardiovascular disease diagnostics.