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Tumor Banks: A Quality Control Scheme Proposal.

Ligia Craciun1,2, Selim Alex Spinette1,2, Marc Rassy2

  • 1Tumor Bank, Institut Jules Bordet, Brussels, Belgium.

Frontiers in Medicine
|November 5, 2019
PubMed
Summary

Implementing robust quality control in tumor banks ensures high-quality frozen samples for translational research, aiding in diagnostics and molecular test development.

Keywords:
DNARNAbiobankelectrophoretic integritymorphologyquality controlquality scores

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Area of Science:

  • Biomedical research
  • Translational oncology
  • Biobanking

Background:

  • Tumor banks are crucial for translational research, enabling new diagnostic and therapeutic strategies.
  • Standardized quality control (QC) for frozen samples in tumor banks is currently lacking in scientific literature.
  • Emerging molecular tests require high-quality biomaterials for reliable results.

Purpose of the Study:

  • To establish and validate a robust quality control framework for fresh frozen samples within a tumor bank.
  • To assess the quality of RNA, DNA, and proteins in frozen tumor samples using molecular and histological techniques.
  • To ensure the suitability of banked samples for advanced molecular testing and biomarker discovery.

Main Methods:

  • Conducted a QC test on 100 fresh frozen samples from the Jules Bordet Tumor Bank.
  • Performed RNA and DNA extraction, evaluating RNA quality via RNA Integrity Number (RIN) and DNA quality via Electrophoretic Integrity (EI).
  • Assessed protein quality using Immunohistochemistry (IHC) for HER2, ER, and Ki67, with a scoring system from 1 (poor) to 4 (excellent).

Main Results:

  • High RNA purity (97% score 4) and RIN scores (89% score 4) were observed.
  • Excellent DNA purity (94% score 4) and EI (100% score 4) were achieved.
  • Immunohistochemistry staining for HER2, ER, and Ki67 yielded a score of 4 in most samples, despite morphology loss.

Conclusions:

  • Tumor banks provide invaluable resources for translational research when high-quality material is ensured.
  • Implementing stringent quality control measures is essential for reliable biobanking.
  • Validated QC protocols enable biobanks to support diagnostics, biomarker discovery, and molecular test development.