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Urine Proteomics Profiling and Functional Characterization of Knee Osteoarthritis Using iTRAQ Technology
Ke Xiao1,2, Lingjia Yu3, Lisi Zhu4
1Department of Orthopedic Surgery, Peking Union Medical College Hospital, Beijing, China.
Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|November 5, 2019
Summary
This study identified 102 urine proteins altered in osteoarthritis patients, including COL-4, MMP9, adiponectin, and BBOX1, offering potential biomarkers for early diagnosis and treatment.
Area of Science:
- Biochemistry
- Proteomics
- Biomarker Discovery
Background:
- Osteoarthritis (OA) is a prevalent degenerative joint disease causing pain and functional loss.
- Aging populations and increased life expectancy exacerbate the socioeconomic burden of OA.
- Current early diagnostic laboratory tests for OA are insufficient, necessitating new methods.
Purpose of the Study:
- To investigate the pathogenesis of OA through comparative urine proteomics.
- To identify potential urinary biomarkers for early OA diagnosis.
- To establish a theoretical basis for early OA treatment strategies.
Main Methods:
- Comparative urine proteomics analysis using iTRAQ-based mass spectrometry.
- Analysis of urine samples from OA patients and healthy individuals.
- Validation of candidate protein biomarkers using Western blotting.
Main Results:
- Identified 102 differentially abundant proteins in OA patient urine.
- 46 proteins were upregulated and 56 were downregulated in OA patients.
- COL-4, MMP9, adiponectin, and BBOX1 were validated as potential OA biomarkers.
Conclusions:
- The identified proteins may serve as valuable biomarkers for early OA diagnosis.
- These findings contribute to understanding OA pathogenesis.
- Provides a foundation for developing early therapeutic interventions for OA.
