Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering
Susanne Pauline Roth1,2, Walter Brehm3, Claudia Groß4
1Faculty of Veterinary Medicine, Veterinary Teaching Hospital, Department for Horses, University of Leipzig, D 04103 Leipzig, Germany. Susanne.roth@uni-leipzig.de.
International Journal of Molecular Sciences
|November 6, 2019
Summary
Transforming growth factor beta 3 (TGFβ3) loaded onto tendon scaffolds maintains its bioactivity. This approach shows promise for enhancing tendon regeneration by preserving TGFβ3
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Cell Biology
Background:
- Transforming growth factor beta 3 (TGFβ3) is known to promote tenogenic differentiation.
- Tendon regeneration is a significant challenge in musculoskeletal medicine.
Purpose of the Study:
- To investigate the bioactivity of TGFβ3 absorbed in decellularized equine superficial digital flexor tendon scaffolds.
- To assess the feasibility of using scaffold-associated TGFβ3 for potential tendon repair applications.
Main Methods:
- TGFβ3 was loaded onto decellularized equine tendon scaffolds.
- Equine adipose tissue-derived multipotent mesenchymal stromal cells (MSCs) were seeded onto TGFβ3-loaded scaffolds.
- Cell morphology, scaffold contraction, and gene expression were analyzed.
Main Results:
- Over 88% of loaded TGFβ3 remained on the scaffolds.
- Scaffold-associated TGFβ3 promoted MSC elongation and scaffold contraction, similar to dissolved TGFβ3.
- Both forms of TGFβ3 modulated MSC gene expression, upregulating tenascin c and downregulating decorin.
Conclusions:
- The bioactivity of TGFβ3 is preserved when absorbed onto decellularized tendon scaffolds.
- Applying TGFβ3 via these scaffolds is a feasible strategy for tendon regeneration.
- This method holds potential for improving outcomes in tendon repair therapies.
