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Updated: Jan 4, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
Lactic acid production by loofah-immobilized Rhizopus oryzae through one-step fermentation process using starch
Sahar Zandkhaneh Shahri1, Farzaneh Vahabzadeh2, Azadeh Mogharei1
1Food Engineering and Biotechnology Group, Chemical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), 424 Hafez Ave., Tehran, Iran.
Abstract:
Rhizopus oryzae PTCC 5263 capacity in synthesis of lactic acid (LA) from 10 g/l of soluble potato starch was determined using one-step fermentation process. Pellets were the favorable growing form of the free cells. The extent of the natural ability of the test fungus on biofilm formation on loofah sponge was examined by immobilizing R. oryzae (LIRO). The maximum LA concentration for the free cells and LIRO within 96 h was 3 and 4 g/l, respectively. In terms of specific starch utilization rate ([Formula: see text]) and specific LA formation ([Formula: see text]), LIRO performed more favorably compared to the free cells ([Formula: see text] and [Formula: see text]). Cell immobilization strategy was undertaken for the column reactor studies based on the statistically optimized levels of the inoculum size and temperature. Maximum production of the LA by the LIRO using an airlift reactor with net draft tube was 5 g/l obtainable within 48 h.
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