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Updated: Jan 4, 2026

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Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
Published on: March 11, 2021
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Nanomolar detection of adenosine triphosphate (ATP) using a nanostructured fluorescent chemosensing ensemble
Ling-Xi Huang1, Qing Guo1, Yong Chen2
1Department of Chemistry, Nanchang University, Nanchang 330031, P. R. China. cqyong@ncu.edu.cn.
Abstract:
We report a novel nanostructured chemosensing ensemble PyNp-C13/UD, obtained by self-assembling uranine dye (UD) and an amphiphilic pyridinium salt PyNp-C13. The ensemble was developed for the fluorescence turn-on sensing of ATP in aqueous solutions and inside living cells. The assembly operates via an indicator displacement assay (IDA) method with an ultra-low detection limit of 6.8 nM.

