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ABILITY TO FORM BIOFILMS BY PYELONEPHRITIS CAUSATIVE AGENTS IN CHILDREN
М Mishyna1, I Marchenko1, S Malanchuk2
1Kharkiv National Medical University, 1D.P. Grynyov Department of Microbiology, Virology and Immunology.
Georgian Medical News
|November 6, 2019
Summary
This study investigated how common childhood pyelonephritis bacteria form biofilms in vitro. Findings reveal distinct biofilm structures for each bacterial type, explaining chronic infection patterns.
Area of Science:
- Microbiology
- Pediatric Nephrology
- Medical Mycology
Background:
- Pyelonephritis in children often involves recurrent infections.
- Bacterial biofilms are implicated in persistent and chronic urinary tract infections.
- Understanding biofilm formation is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the in vitro biofilm formation process of key pyelonephritis pathogens in children.
- To characterize the structural and morphological differences in biofilms produced by various bacterial species.
- To correlate biofilm characteristics with the pathogenesis of chronic pyelonephritis and relapses.
Main Methods:
- In vitro cultivation of pyelonephritis causative agents in polystyrene Petri dishes.
- Microscopic analysis including light, fluorescent, and scanning electron microscopy.
- Observation of bacterial adhesion, matrix formation, and biofilm maturation stages.
Main Results:
- All studied bacterial isolates, including E. coli, Proteus spp., P. aeruginosa, K. pneumoniae, and E. faecalis, formed biofilms.
- Distinct biofilm architectures were observed for each bacterial species, characterized by specific cell arrangements and matrix compositions.
- P. aeruginosa biofilms exhibited a dense, gel-like structure, while K. pneumoniae biofilms showed a matrix with canals. E. faecalis biofilms had densely packed cells.
- Biofilm formation involves sequential stages: bacterial adhesion, matrix production, and maturation.
Conclusions:
- Bacterial biofilms are formed by conglomerates of cells embedded in an extracellular matrix.
- The specific nature of the biofilm is dependent on the bacterial species.
- The formation of these diverse biofilms contributes to the chronic and relapsing nature of pyelonephritis in children.
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