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Long-term sperm cryopreservation does not affect post-thaw survival rates
Juliana R Pariz1,2,3,4, Rosa Alice C Monteiro1, Jorge Hallak1,2,3,4
1Androscience, Science and Innovation Center in Andrology and High-Complex Clinical and Andrology Laboratory. São Paulo, Brazil.
JBRA Assisted Reproduction
|November 6, 2019
Summary
Human sperm cryosurvival rates remain stable after prolonged cryopreservation. A method removing seminal debris ensures consistent sperm quality for assisted reproduction, even after 3-12 years.
Area of Science:
- Reproductive medicine
- Cryobiology
Background:
- Sperm cryopreservation is crucial for assisted reproduction.
- Long-term cryopreservation may impact sperm viability.
Purpose of the Study:
- To evaluate human spermatozoa cryosurvival rates after prolonged cryopreservation (3-12 years).
Main Methods:
- Retrospective analysis of 33 cancer patient semen samples cryopreserved between 2002-2011.
- Sperm cryosurvival rate (CS) calculated using post-thaw motility.
- Comparison between samples cryopreserved for 24 hours, 3-12 years, and a validation group.
Main Results:
- No significant difference in cryosurvival rates was observed between samples cryopreserved for 24 hours (26.11±46.36%) and those cryopreserved for 3-12 years (23.71±57.06%).
- The validation group also showed comparable cryosurvival rates.
- Initial semen analysis showed no significant differences between study and validation groups.
Conclusions:
- The cryopreservation method, which includes pre-freezing seminal debris removal, effectively maintains human sperm cryosurvival rates over extended periods.
- This technique ensures consistent sperm quality for assisted reproduction procedures.
