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hsa_circ_0092306 Targeting miR-197-3p Promotes Gastric Cancer Development by Regulating PRKCB in MKN-45 Cells.

Zihao Chen1, Hongping Ju2, Ting Zhao3

  • 1Graduate School of Hebei Medical University, Shijiazhuang 050017, Hebei, China; The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China.

Molecular Therapy. Nucleic Acids
|November 6, 2019
PubMed
Summary
This summary is machine-generated.

Circular RNA hsa_circ_0092306 promotes gastric cancer (GC) progression by upregulating protein kinase C beta (PRKCB) via inhibiting miR-197-3p. This study identifies hsa_circ_0092306 as a potential therapeutic target for gastric cancer.

Keywords:
PRKCBcircular RNAgastric cancerhsa_circ_0092306miR-197-3pprotein kinase C beta

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer (GC) represents a significant global health challenge.
  • Circular RNAs (circRNAs) are emerging as key regulators in various cancers, including GC.
  • Understanding the molecular mechanisms of circRNAs in GC is crucial for developing novel therapeutic strategies.

Purpose of the Study:

  • To investigate the role and molecular mechanism of the novel circRNA hsa_circ_0092306 in gastric cancer (GC).
  • To explore hsa_circ_0092306 as a potential therapeutic target for GC treatment.

Main Methods:

  • Bioinformatic analysis of GEO microarray data.
  • Validation of hsa_circ_0092306 expression and identification using Sanger sequencing and RNase R assay.
  • Quantitative real-time PCR and Western blot for mRNA and protein level analysis.
  • Functional assays including apoptosis, viability, migration, and invasion assays in MKN-45 cells.
  • In vivo xenograft experiments to confirm findings.

Main Results:

  • hsa_circ_0092306 was significantly upregulated in GC tissues and cells.
  • hsa_circ_0092306 promoted GC cell proliferation, migration, and invasion.
  • hsa_circ_0092306 was found to inhibit the tumor suppressor miR-197-3p and promote the oncogenic protein kinase C beta (PRKCB).
  • The miR-197-3p/PRKCB pathway was identified as a key mechanism regulated by hsa_circ_0092306.

Conclusions:

  • hsa_circ_0092306 plays a crucial role in promoting gastric cancer development.
  • The regulatory pathway involving hsa_circ_0092306, miR-197-3p, and PRKCB offers a novel therapeutic target for gastric cancer.