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Intramolecular platinum migration on a peptide in gas phase during collision-induced dissociation
Zhifeng Du1, Yun Qu2, Nicholas P Farrell2
1Department of Chemistry, Virginia Commonwealth University, Richmond, VA 23284-2006, USA; Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Abstract:
We report the migration of platinum ligand unit {Pt(en)}2 (en = ethylenediamine) on a short peptide during collision-induced dissociation fragmentation combined with the characterization of the same species by 2D [1H,15N] HSQC (Heteronuclear Single Quantum Coherence) NMR spectroscopy. The NMR spectrum showed that the cysteine is platinated while the MS/MS (Tandem mass spectrometry) showed the platination at glutamic acid. Our results provide the first experimental evidence of platinum migration on peptide during collision-induced dissociation.
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