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Human serum lipoamidase.

K Hayakawa1, J Oizumi

  • 1National Children's Medical Research Center, Division of Metabolism, Tokyo, Japan.

Enzyme
|January 1, 1988
PubMed
Summary

Human serum contains lipoamidase (lipoyl-4-aminobenzoate hydrolase) activity, with optimal function at pH 7.0. This enzyme shows a strong correlation with biotinidase activity, suggesting a potential role in lipoate recycling.

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Area of Science:

  • Biochemistry
  • Enzymology
  • Clinical Chemistry

Background:

  • Lipoamidase (lipoyl-4-aminobenzoate hydrolase) is an enzyme found in human serum.
  • Its physiological role remains largely unknown.
  • Biotinidase plays a crucial role in biotin metabolism and recycling.

Purpose of the Study:

  • To characterize human serum lipoamidase activity.
  • To investigate the correlation between lipoamidase and biotinidase activities in children.
  • To explore the potential physiological significance of serum lipoamidase.

Main Methods:

  • Serum samples from 32 individuals aged 1-8 years were analyzed.
  • A newly synthesized substrate, lipoyl-4-aminobenzoate, was used to assay lipoamidase activity.
  • High-performance liquid chromatography (HPLC) with fluorimetric detection was employed for product quantification.
  • Biotinidase activity was also measured in the same samples.

Main Results:

  • All tested human sera exhibited lipoamidase activity.
  • The optimal pH for serum lipoamidase was determined to be 7.0.
  • Enzyme activity saturated at a substrate concentration of 0.1 mmol/l.
  • Mean serum lipoamidase activity was 1.50 U/l (SD 1.037) in children aged 1-8 years.
  • No significant sex-based difference in activity was observed.
  • A strong positive correlation (r = 0.8931) was found between lipoamidase and biotinidase activities.

Conclusions:

  • Human serum possesses significant lipoamidase activity.
  • The strong correlation with biotinidase suggests lipoamidase may be involved in lipoate recycling.
  • Further research is warranted to elucidate the precise physiological function of serum lipoamidase.

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