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Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
Published on: July 11, 2012
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Layer by Layer Antimicrobial Coatings Based on Nafion, Lysozyme, and Chitosan
Ella N Gibbons1, Charis Winder2, Elliot Barron3
1School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston PR1 2HE, UK. engibbons3@uclan.ac.uk.
Nanomaterials (Basel, Switzerland)
|November 7, 2019
Summary
This study developed novel Nafion-based coatings with lysozyme and chitosan, effectively eliminating nearly 100% of Escherichia coli and Staphylococcus aureus bacteria. These synergistic platforms offer advanced antimicrobial defense beyond simple exclusion.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Microbiology
Background:
- Microbial contamination poses significant challenges in various applications.
- Developing effective antimicrobial surfaces is crucial for preventing infections and material degradation.
Purpose of the Study:
- To engineer a novel layer-by-layer coating using Nafion, lysozyme, and chitosan.
- To investigate the molecular interactions and surface properties of these nanocoatings.
- To evaluate the antimicrobial efficacy against common bacterial strains.
Main Methods:
- Layer-by-layer assembly monitored by Quartz Crystal Microbalance.
- Molecular interactions studied using Circular Dichroism.
- Surface characterization via Atomic Force Microscopy, contact angle, and zeta potential measurements.
Main Results:
- Successful fabrication of Nafion, lysozyme, and chitosan multilayer coatings.
- Demonstrated strong molecular interactions between coating components.
- Achieved nearly 100% reduction in Escherichia coli and Staphylococcus aureus populations on nanocoated surfaces.
Conclusions:
- Nafion-based synergistic coatings present a highly effective antimicrobial strategy.
- These platforms offer robust defense mechanisms against bacterial contamination.
- The developed coatings show potential for broad applications requiring antimicrobial properties.
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