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Expression-Based Cell Lineage Analysis in Drosophila Through a Course-Based Research Experience for Early
John M Olson1, Cory J Evans2,3, Kathy T Ngo1
1Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095.
G3 (Bethesda, Md.)
|November 7, 2019
Summary
The G-TRACE system visualizes cell lineages and gene expression in Drosophila, revealing new developmental patterns. This discovery-based research program significantly enhanced student learning and STEM retention.
Area of Science:
- Developmental Biology
- Genetics
- Genomics
Background:
- Cell lineage tracing is crucial for understanding tissue development.
- Existing methods often do not correlate lineage with gene expression.
- The G-TRACE system enables visualization of past and present gene expression patterns linked to cell lineages.
Purpose of the Study:
- To perform a genome-wide, expression-based lineage screen using the G-TRACE system.
- To identify novel cell lineage patterns in Drosophila development.
- To assess the educational impact of a discovery-based research program.
Main Methods:
- Utilized the G-TRACE system for fluorescent protein-based lineage tracing.
- Conducted a genome-wide screen involving undergraduate and high school students.
- Compiled findings into the G-TRACE Expression Database (GED).
Main Results:
- Discovered novel expression-based lineage patterns in Drosophila brain, imaginal disc epithelia, and hematopoietic lymph gland.
- Established the G-TRACE Expression Database (GED) as a resource for Drosophila researchers.
- Demonstrated significant learning gains and improved STEM retention for participating students.
Conclusions:
- The G-TRACE system is effective for large-scale, expression-based lineage screens.
- Discovery-based research programs can significantly enhance student education and career persistence in STEM.
- The GED provides valuable data for the Drosophila research community.
