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Updated: Jan 4, 2026
Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis
Published on: June 2, 2023
A mitochondria-targeted fluorescent probe based on coumarin-pyridine derivatives for hypochlorite imaging in living
Xiuli Zhong1, Qing Yang1, Yingshuang Chen1
1Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, P. R. China. 07205@njnu.edu.cn Wangbingxiang@njnu.edu.cn jshen@njnu.edu.cn.
Abstract:
Hypochlorite plays a critical role in various physiological processes and is involved in many diseases. Thus, real-time, rapid, and accurate monitoring of hypochlorite has important medical and physiological significance. Herein, a novel coumarin-pyridine derivative (CPD) probe was designed and synthesized, which exhibited fantastic advantages, such as a rapid response (within 10 s), naked eye recognition, large Stokes shift (185 nm), dual-channel detection, and high selectivity and sensitivity toward OCl- (detection limit 0.012 μM, S/N = 3). Furthermore, the current CPD probe was successfully used to image OCl- in the mitochondria of both A549 cells and zebrafish, which further demonstrated its suitability for practical applications in biological systems.
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