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Blastocystis sp. Subtype Diversity in Wild Carnivore Species from Spain
Rafael Calero-Bernal1, Mónica Santín2, Jenny G Maloney2
1SALUVET, Department of Animal Health, Faculty of Veterinary, Complutense University of Madrid, Av. Puerta de Hierro s/n, 28040, Madrid, Spain.
Abstract:
The occurrence and molecular diversity of the stramenopile eukaryote Blastocystis sp. was investigated by PCR and sequencing (Sanger and NGS) methods in 380 faecal specimens of free-living carnivores in Spain. Blastocystis sp. was confirmed in 1.6% (6/380) of the specimens analysed. Two samples from a common genet and a fox were successfully subtyped as ST7 by Sanger. Using NGS, ST14 was found in a fox and a European polecat, ST7 in a fox, and two additional foxes presented mixed infections of ST1/ST2/ST4 and ST1/ST2/ST7, respectively. Wild carnivore species could act as carriers of zoonotic Blastocystis subtypes.
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