DrLLPS: a data resource of liquid-liquid phase separation in eukaryotes.
Wanshan Ning1, Yaping Guo1, Shaofeng Lin1
1Key Laboratory of Molecular Biophysics of Ministry of Education, Hubei Bioinformatics and Molecular Imaging Key Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Nucleic Acids Research
|November 7, 2019
Summary
DrLLPS is a new database for proteins driving liquid-liquid phase separation (LLPS), a key process in forming cellular compartments. It catalogs over 437,000 proteins across eukaryotes, aiding LLPS research.
Area of Science:
- Biochemistry and Molecular Biology
- Cell Biology
- Bioinformatics
Background:
- Liquid-liquid phase separation (LLPS) is essential for organizing cellular reactions via membraneless organelles (MLOs).
- Understanding LLPS is crucial for comprehending cellular function and dysfunction.
Purpose of the Study:
- To present DrLLPS, an integrated database for proteins involved in LLPS.
- To provide a comprehensive resource for studying LLPS-associated proteins and their roles.
Main Methods:
- Manual literature curation of scaffold, regulator, and client proteins involved in LLPS.
- Identification of orthologs for known LLPS proteins across eukaryotes.
- Integration of diverse biological data including PTMs, mutations, interactions, and expression data.
Main Results:
- DrLLPS database contains 437,887 known and potential LLPS-associated proteins from 164 eukaryotes.
- Proteins are categorized into 40 biomolecular condensates.
- Detailed annotations cover 16 aspects, including functional, structural, and disease-related information for proteins in eight model organisms.
Conclusions:
- DrLLPS serves as a valuable, comprehensive resource for researchers investigating the mechanisms and implications of LLPS.
- The database facilitates further analysis of LLPS-associated proteins and their functions in various biological contexts.
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