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Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
Published on: December 17, 2015
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Distinct gene expression characteristics in epithelial cell-Porphyromonas gingivalis interactions by integrating
Dongmei Zhang1, Jingya Hou2, Yun Wu2
1Department of Periodontics and Oral Biology, School of Stomatology, China Medical University, Shenyang 110002, China.
International Journal of Medical Sciences
|November 7, 2019
Summary
Porphyromonas gingivalis infection up-regulates genes in epithelial cells, promoting survival and proliferation. This may contribute to periodontitis and oral cancer development through inflammation and altered cell regulation.
Area of Science:
- Microbiology
- Genomics
- Oral Biology
Background:
- Porphyromonas gingivalis is a key pathogen in periodontitis.
- Epithelial cells form the primary defense against bacterial invasion.
- Host-bacteria interactions influence disease pathogenesis.
Purpose of the Study:
- To identify differential gene expression profiles in epithelial cells upon Porphyromonas gingivalis infection.
- To analyze gene ontology and pathway enrichment of differentially expressed genes.
Main Methods:
- A network-based meta-analysis of gene expression profiling was employed.
- Gene ontology analysis was performed on differentially expressed genes.
- Pathway enrichment analysis identified affected biological pathways.
Main Results:
- 290 genes were consistently up-regulated in Porphyromonas gingivalis-infected epithelial cells.
- Up-regulated genes were associated with "negative regulation of apoptotic process" and "positive regulation of cell proliferation/migration/angiogenesis".
- Pathways linked to inflammation and "transcriptional misregulation in cancer" were enriched.
Conclusions:
- Porphyromonas gingivalis infection enhances epithelial cell survival and proliferation, aiding colonization.
- Chronic inflammation and altered gene regulation by P. gingivalis may contribute to oral cancer development.
- Negative regulation of apoptosis and cancer-related pathways are crucial in periodontitis and oral cancer pathogenesis.
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