Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jan 4, 2026

Sample Preparation for Mass Cytometry Analysis
06:28

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

16.5K

Signal Amplification for Imaging Mass Cytometry.

Rahul Rana1, Rodolfo F Gómez-Biagi2, Jay Bassan1,3

  • 1Department of Chemistry , University of Toronto , 80 St. George Street , Toronto , ON M5S 3H6 , Canada.

Bioconjugate Chemistry
|November 7, 2019
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

OC43 clinical isolate spike proteins have distinct carbohydrate-binding properties.

Nature communications·2026
Same author

Protease-triggered self-immolative acyl phosphates for controlled phosphate release.

Organic & biomolecular chemistry·2026
Same author

Tellurophene-Tagged Carfilzomib Enables Single-Cell Mass Cytometric Mapping of Proteasome Activity.

ACS chemical biology·2025
Same author

Human coronavirus HKU1 spike structures reveal the basis for sialoglycan specificity and carbohydrate-promoted conformational changes.

Nature communications·2025
Same author

Exploration of Glycosyl Dithioimidocarbonates in Photoinduced Desulfurative Cross-Coupling Reactions.

Organic letters·2024
Same author

Oxidation-Controlled, Strain-Promoted Tellurophene-Alkyne Cycloaddition (OSTAC): A Bioorthogonal Tellurophene-Dependent Conjugation Reaction.

Journal of the American Chemical Society·2024
Same journal

Neutral Amphiphiles Boost Transfection Efficiency and Reduce Inflammation in Polymer Micelle-Mediated mRNA Delivery.

Bioconjugate chemistry·2026
Same journal

Surfactant-Mediated Buchwald-Hartwig Coupling of Aliphatic Amines for the Synthesis of DNA-Encoded Libraries.

Bioconjugate chemistry·2026
Same journal

Artificial Intelligence for Discovery in Life Sciences.

Bioconjugate chemistry·2026
Same journal

Iron Single Atom Nanozyme-Mediated GPX4 Inhibitor Delivery for Self-Enhanced Ferroptosis.

Bioconjugate chemistry·2026
Same journal

SpyCatcher-Engineered Ferritin Nanocages Enable Dual-Receptor Targeting for Enhanced Glioma Therapy.

Bioconjugate chemistry·2026
Same journal

One-Pot Synthesis of Functionalized Coumarin Fluorophores Enables Rapid Access to Live-Cell Bioorthogonal Labeling and Microenvironmental Sensing Agents.

Bioconjugate chemistry·2026
See all related articles

A new enzyme-catalyzed reporter deposition stain enhances Imaging Mass Cytometry (IMC) by amplifying signals for low-abundance biomarker detection. This method complements existing antibody conjugates for multiparametric antigen analysis.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cellular Imaging

Background:

  • Imaging Mass Cytometry (IMC) is a powerful technique for high-parameter single-cell analysis.
  • Current methods for IMC often rely on heavy isotope-conjugated antibodies, which can be limited in sensitivity for detecting low-abundance biomarkers.
  • There is a need for complementary strategies to enhance signal detection and expand the capabilities of IMC.

Purpose of the Study:

  • To develop a novel enzyme-catalyzed reporter deposition stain for IMC.
  • To enable signal amplification for detecting low-abundance biomarkers.
  • To integrate this new method with existing IMC workflows for multiparametric antigen detection.

Main Methods:

  • Development of an alkaline phosphatase substrate tethered to a tellurophene reporter group.

More Related Videos

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry
06:51

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Published on: November 14, 2025

244
Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
06:47

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

2.5K

Related Experiment Videos

Last Updated: Jan 4, 2026

Sample Preparation for Mass Cytometry Analysis
06:28

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

16.5K
Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry
06:51

Dual-modality Molecular Cartography: Integrating Multiplex mRNA Detection with Protein Imaging Mass Cytometry

Published on: November 14, 2025

244
Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
06:47

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

2.5K
  • Utilizing enzyme-catalyzed hydrolysis to release a reactive intermediate that labels local nucleophiles.
  • Integration of the reporter deposition stain with standard IMC antibody staining protocols.

    • Main Results:

    • Successful development of an enzyme-catalyzed reporter deposition stain for IMC.
    • Demonstrated signal amplification capabilities, particularly for low-abundance biomarkers.
    • Validated seamless integration with standard IMC antibody staining for multiparametric analysis.

    Conclusions:

    • The developed reporter deposition stain is a valuable addition to IMC techniques.
    • This strategy effectively complements heavy isotope antibody conjugates by enhancing signal detection.
    • The method facilitates sensitive and multiparametric antigen detection in IMC applications.