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Separation of Crassostrea gigas hemocytes by density gradient centrifugation and counterflow centrifugal elutriation
E Bachère1, D Chagot, H Grizel
1IFREMER, Laboratoire de pathologie et de Génétique des Invertébrés Marins, La Tremblade, France.
A protocol is described to separate several subpopulations of hemocytes in a unique medium which avoids cell aggregation and retains cell-viability. Isopycnic centrifugation in Percoll followed by counterflow centrifugal elutriation provides large quantities of separated granulocyte and hyalinocyte subpopulations.
A protocol is described to separate several subpopulations of hemocytes in a unique medium which avoids cell aggregation and retains cell-viability. Isopycnic centrifugation in Percoll followed by counterflow centrifugal elutriation provides large quantities of separated granulocyte and hyalinocyte subpopulations.