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E2FB Interacts with RETINOBLASTOMA RELATED and Regulates Cell Proliferation during Leaf Development
Erika Őszi1,2, Csaba Papdi3, Binish Mohammed3
1Institute of Plant Biology, Biological Research Centre, Szeged, 6726, Hungary.
Plant Physiology
|November 8, 2019
Summary
E2F/E2FB transcription factors and RETINOBLASTOMA-RELATED (RBR) proteins regulate plant cell proliferation. This study reveals E2FB
Area of Science:
- Plant Molecular Biology
- Developmental Biology
- Cell Cycle Regulation
Background:
- E2F transcription factors, alongside RETINOBLASTOMA-RELATED (RBR) proteins, are key regulators of cell cycle entry and quiescence.
- The specific role of E2F/E2FB during plant development, particularly in leaf development, is not well understood.
- While often viewed as transcriptional activators, E2FB's function in conjunction with RBR requires further elucidation.
Purpose of the Study:
- To investigate the function of E2FB in association with RBR during Arabidopsis leaf development.
- To determine how E2FB-RBR interactions influence cell proliferation and quiescence establishment.
- To understand the regulatory mechanisms controlling leaf cell number and organogenesis.
Main Methods:
- Analysis of E2FB-RBR protein interactions.
- Quantitative assessment of E2F target gene expression.
- Microscopic examination of epidermal cell number and shape in Arabidopsis mutants and overexpression lines.
- Study of leaf development across different stages.
Main Results:
- E2FB, in complex with RBR, acts as a repressor of cell proliferation, promoting quiescence during early leaf development.
- Elevated E2FB levels enhance the repressor complex, reducing leaf cell proliferation and final leaf cell number.
- Mutations in E2FB or overexpression of a non-RBR-binding E2FB mutant lead to cell overproliferation, highlighting RBR's specific role via E2FB.
- Meristemoid cells show preferential hyperproliferation in e2fb mutants.
- During later stages, declining RBR and E2FB levels allow E2FB to reactivate cell division.
Conclusions:
- E2FB functions with RBR to inhibit cell proliferation and establish quiescence, critically determining final leaf cell number in Arabidopsis.
- The E2FB-RBR complex acts as a central regulator of cell proliferation during organ development.
- Understanding this complex is crucial for dissecting the genetic control of plant organ size and development.
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