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AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection
Weijie Wu1, Xun Wang1, Mengfei Shen1
1State Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
Theranostics
|November 8, 2019
Summary
This study introduces a novel suspension array platform using stable AIEgens (Aggregation-Induced Emissiongens) microbeads and nanobeads for enhanced multiplexed bioassays. The platform significantly improves detection sensitivity for precision diagnostics.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Materials Science
Background:
- Suspension arrays are crucial for multiplexed detection in various fields like gene analysis and disease diagnosis.
- Current limitations include insufficient fluorescence stability and detection sensitivity, hindering precision diagnostics.
Purpose of the Study:
- To develop a novel suspension array platform with enhanced fluorescence stability and detection sensitivity.
- To utilize AIEgens (Aggregation-Induced Emissiongens) for improved multiplexed bioassays.
Main Methods:
- Developed a suspension array platform using AIEgens microbeads (AIE33, AIE NIR800) as stable barcodes.
- Employed AIEgens nanobeads (HPS) as fluorescent signal reporters.
- Utilized flow cytometry for multiplexed detection.
Main Results:
- The AIEgens nanobeads provided excellent fluorescent signal amplification.
- Achieved up to 3-fold and 5-fold sensitivity improvement compared to QDs and phycoerythrin reporters, respectively.
- Demonstrated comparable performance to the ImmunoCAP method for allergen detection in patient serum.
Conclusions:
- The AIEgens-based suspension array platform offers excellent fluorescence stability and signal amplification.
- This platform shows promise for high-sensitivity multiplexed bioassay applications in precision diagnostics.
