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Updated: Jan 4, 2026
10:55
Purification of Ubiquitinated p53 Proteins from Mammalian Cells
Published on: March 21, 2022
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A negative feedback regulatory loop between miR-138 and TP53 is mediated by USP10
Zhenghua Luo1, Manchao Zhang1, Ri Cui1
1Department of Cancer Biology and Medical Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.
Oncotarget
|November 8, 2019
Summary
MicroRNA-138 regulates the tumor suppressor TP53 by targeting USP10. A feedback loop exists where TP53 represses microRNA-138, adding complexity to cancer gene networks.
Area of Science:
- Molecular Biology
- Cancer Research
- Gene Regulation
Background:
- TP53 is a crucial tumor suppressor gene, frequently altered in human cancers.
- TP53 expression is tightly regulated by pathways including Mdm2-mediated ubiquitination and deubiquitination by USP7 and USP10.
- MicroRNAs play roles in regulating TP53 levels and function.
Purpose of the Study:
- To investigate the role of microRNA-138 in regulating TP53.
- To elucidate the regulatory mechanism involving microRNA-138, TP53, and USP10.
Main Methods:
- Investigated microRNA-138's direct targeting of USP10.
- Analyzed the feedback loop between TP53 and microRNA-138 expression.
Main Results:
- MicroRNA-138 directly targets and regulates USP10.
- TP53 was found to repress microRNA-138 expression.
- A negative feedback regulatory loop between TP53 and microRNA-138 was identified.
Conclusions:
- MicroRNA-138 acts as a regulator of TP53 by targeting USP10.
- The discovered negative feedback loop adds complexity to the TP53 regulatory network in cancer.
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