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Updated: Jan 4, 2026
3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
Published on: September 13, 2018
MiR-652-3p promotes bladder cancer migration and invasion by targeting KCNN3
1Department of Urology Surgery, Jiangdu People's Hospital of Yangzhou, Yangzhou, P.R. China. qingliang_zhu@126.com.
Objective:
Increasing evidence indicated that microRNAs (miRNAs) are crucial regulators for cancer development. Bladder cancer (BCa) is a major threat to human health. The aim of this study was to analyze the roles of miR-652-3p in BCa, and to explore the associated mechanisms.
Materials And Methods:
MiR-652-3p expression in BCa cell lines was explored using Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) method. MiR-652-3p expression level in BCa tissues was explored at StarBase. Cell Counting Kit-8 (CCK-8) assay, wound-healing assay, and transwell invasion assay were conducted to investigate the biological roles of miR-652-3p. The underlying mechanisms of miR-652-3p in NSCLC were investigated using luciferase activity reporter assay and rescue experiments.
Results:
We showed that miR-652-3p expression level was upregulated in both BCa tissues and cell lines. The knockdown of miR-652-3p significantly inhibited BCa cell proliferation, migration, and invasion in vitro. Moreover, we showed that potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3 (KCNN3) was a functional target for miR-652-3p. Besides, the expression of KCNN3 in BCa tissues was negatively correlated with miR-652-3p.
Conclusions:
Collectively, these results showed that miR-652-3p could promote BCa cell proliferation, migration, and invasion via directly regulating KCNN3, which may provide a novel therapeutic target for BCa treatment.
Insights
MicroRNA miR-652-3p promotes bladder cancer (BCa) progression by upregulating KCNN3. Targeting miR-652-3p offers a potential therapeutic strategy for BCa treatment.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- MicroRNAs (miRNAs) are key regulators in cancer development.
- Bladder cancer (BCa) poses a significant global health challenge.
- Understanding specific miRNA roles is crucial for BCa research.
Purpose of the Study:
- To investigate the role of miR-652-3p in bladder cancer.
- To elucidate the underlying molecular mechanisms of miR-652-3p in BCa.
- To identify potential therapeutic targets for BCa.
Main Methods:
- Real-Time quantitative Polymerase Chain Reaction (RT-qPCR) for miRNA expression analysis.
- In vitro assays (CCK-8, wound-healing, transwell invasion) to assess cell behavior.
- Luciferase reporter assays and rescue experiments to determine molecular targets and mechanisms.
Main Results:
- miR-652-3p expression was significantly upregulated in BCa tissues and cell lines.
- Downregulation of miR-652-3p suppressed BCa cell proliferation, migration, and invasion.
- KCNN3 was identified as a direct functional target of miR-652-3p, with inverse correlation in BCa tissues.
Conclusions:
- miR-652-3p promotes bladder cancer cell proliferation, migration, and invasion by directly regulating KCNN3.
- miR-652-3p represents a potential novel therapeutic target for bladder cancer treatment.
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