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LncRNA AOC4P affects biological behavior of gastric cancer cells through MAPK signaling pathway

C-X Qu1, X-C Shi, H Bi

  • 1Department of Pathology, Shanxi Provincial People's Hospital, Taiyuan, Shanxi, China. chongxiao521@163.com.

European Review for Medical and Pharmacological Sciences
|November 8, 2019
PubMed
Abstract

Insights

High expression of long non-coding RNA (lncRNA) AOC4P correlates with gastric cancer progression and poor prognosis. Inhibiting lncRNA AOC4P may offer a new therapeutic strategy for gastric cancer patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play a role in cancer development.
  • The specific role and clinical significance of lncRNA AOC4P in gastric cancer remain largely unknown.

Purpose of the Study:

  • To investigate the expression levels and functional role of lncRNA AOC4P in gastric cancer.
  • To explore the potential of lncRNA AOC4P as a diagnostic marker and therapeutic target.

Main Methods:

  • Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) to assess lncRNA AOC4P expression in 80 gastric cancer tissues and adjacent normal tissues.
  • In vitro assays including MTT, flow cytometry, and Transwell assays to evaluate the impact of lncRNA AOC4P on gastric cancer cell proliferation, apoptosis, migration, and invasion.
  • Western blot analysis to examine the involvement of the mitogen-activated protein kinase (MAPK) signaling pathway.

Main Results:

  • lncRNA AOC4P was significantly upregulated in gastric cancer tissues compared to adjacent normal tissues.
  • High lncRNA AOC4P expression was associated with shorter Overall Survival (OS) and Disease-Free Survival (DFS) times.
  • Inhibition of lncRNA AOC4P suppressed cell proliferation, migration, and invasion, while promoting apoptosis.
  • Downregulation of lncRNA AOC4P led to decreased expression of key MAPK pathway proteins: ERK1, JNK, and p38.

Conclusions:

  • Elevated lncRNA AOC4P expression is implicated in the occurrence, development, and poor prognosis of gastric cancer.
  • lncRNA AOC4P presents potential as a novel diagnostic biomarker and therapeutic target for gastric cancer.

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