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MiR-181d inhibits cell proliferation and metastasis through PI3K/AKT pathway in gastric cancer

K Jiang1, L-F Xie, T-Z Xiao

  • 1General Surgery, Wuxi No. 4 People's Hospital, Affiliated Hospital of Jiangnan University, Wuxi, China. drraphaeljk@126.com.

European Review for Medical and Pharmacological Sciences
|November 8, 2019
PubMed
Abstract

Insights

MicroRNA-181d (miR-181d) acts as a tumor suppressor in gastric cancer, inhibiting proliferation and invasion. Its downregulation correlates with poor patient prognosis, highlighting its therapeutic potential.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer is a leading cause of cancer mortality globally.
  • MicroRNA-181d (miR-181d) is recognized as a tumor suppressor in various cancers.
  • The specific role of miR-181d in gastric cancer requires further elucidation.

Purpose of the Study:

  • To investigate the functional significance of miR-181d in gastric cancer.
  • To determine the relationship between miR-181d expression and patient prognosis.
  • To identify the molecular mechanisms underlying miR-181d's action in gastric cancer.

Main Methods:

  • Real-Time quantitative Polymerase Chain Reaction (RT-qPCR) and Western blot for gene and protein expression analysis.
  • MTT and Transwell assays to assess cell proliferation and invasion.
  • Kaplan-Meier analysis for overall survival prediction.
  • In vivo xenograft models to evaluate tumor growth suppression.

Main Results:

  • miR-181d was significantly downregulated in gastric cancer tissues and cell lines.
  • Lower miR-181d levels were associated with poor patient prognosis.
  • miR-181d directly targeted Cylindromatosis gene (CYLD), inhibiting the CYLD/PI3K/AKT pathway and epithelial-mesenchymal transition (EMT).
  • Overexpression of miR-181d suppressed tumor growth both in vitro and in vivo.

Conclusions:

  • miR-181d functions as a tumor suppressor in gastric cancer by inhibiting proliferation and invasion.
  • The miR-181d/CYLD axis plays a crucial role in gastric cancer pathogenesis.
  • This study offers new insights into gastric cancer development and potential therapeutic strategies targeting the miR-181d/CYLD pathway.

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