An Inexpensive, simple calibration method for MALDI TOF/TOF systems

Area of Science:

• Analytical Chemistry
• Mass Spectrometry
• Biochemistry

Background:

• Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MADLI MS) enables the measurement of a wide array of analytes.
• The extensive range of measurable analytes necessitates a vast number of calibration mixtures.
• Commercial calibration mixtures are often prohibitively expensive, limiting accessibility.

Purpose of the Study:

• To develop a low-cost alternative to commonly used peptide calibrants for MADLI MS.
• To assess the performance of the developed calibrant in terms of mass accuracy.
• To establish a viable and affordable option for MADLI MS calibration.

Main Methods:

• Synthesis of a novel, low-cost peptide calibrant mixture.
• Analysis of the calibrant using MADLI MS.
• Evaluation of mass accuracy across a specified mass range.

Main Results:

• Achieved an overall mass accuracy of 78 ppm.
• Demonstrated comparable mass accuracy to commercial peptide mixes.
• Successful calibration across a mass range of 900 to 2500 Da.

Conclusions:

• The developed low-cost peptide calibrant is a viable alternative to expensive commercial options.
• This provides a cost-effective solution for routine MADLI MS calibration.
• Enables broader application of MADLI MS by reducing calibration costs.