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Removal of half antibody, hole-hole homodimer and aggregates during bispecific antibody purification using MMC ImpRes

Jiaqin Tang1, Xudong Zhang1, Tao Chen1

  • 1Technology and Process Development (TPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.

Protein Expression and Purification
|November 8, 2019
PubMed

During recombinant production of asymmetric IgG-like bispecific antibodies (bsAbs), various by-products are often observed due to unbalanced chain expression and incorrect chain pairing. Among them, half antibody and homodimer are found with high frequency. In this work, with a case study we demonstrated that Capto MMC ImpRes mixed-mode chromatography can effectively remove these two by-products as well as antibody aggregates under optimized conditions. This makes MMC ImpRes a powerful tool for bsAb purification.

Keywords:
Antibody aggregatesBispecific antibody (bsAb)Capto MMC ImpResHalf antibodyHole-hole homodimerMixed-mode chromatography

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