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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
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Resistance and cross-resistance in Staphylococcus spp. strains following prolonged exposure to different antiseptics

Getuţa Nicolae Dopcea1, Ioan Dopcea2, Alina Elena Nanu2

  • 1University of Agronomic Sciences and Veterinary Medicine of Bucharest, Biotechnology Department, Applied Microbiology Laboratory, 59 Mărăști Blvd., District 1, Bucharest, Romania; Eurofins Evic Product Testing Romania SRL, 64-66 Mărășești Blvd., District 4, Bucharest, Romania.

Journal of Global Antimicrobial Resistance
|November 8, 2019
PubMed
Abstract

Insights

Exposure to common antiseptics like chlorhexidine digluconate (CHG) and octenidine dihydrochloride (OCT) can lead to staphylococci developing resistance. This study highlights the need for cautious antiseptic use to prevent antibiotic cross-resistance.

Area of Science:

  • Microbiology
  • Antimicrobial Resistance
  • Public Health

Background:

  • Antiseptics are crucial for infection control.
  • The potential for antiseptics to induce antimicrobial resistance is a growing concern.
  • Staphylococci are common pathogens with varying resistance profiles.

Purpose of the Study:

  • To investigate the development of antiseptic resistance and antibiotic cross-resistance in staphylococci.
  • To evaluate the impact of chlorhexidine digluconate (CHG) and octenidine dihydrochloride (OCT) exposure on staphylococcal resistance.

Main Methods:

  • Determined antibiotic susceptibility and antiseptic minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs).
  • Tested Staphylococcus epidermidis and Staphylococcus aureus clinical and reference strains.
  • Exposed strains to low concentrations of CHG and OCT over an extended period.

Main Results:

  • Resistance to both CHG and OCT was observed after exposure.
  • CHG exposure increased MICs in most tested strains and decreased susceptibility to antibiotics like gentamicin, penicillin, and tetracycline.
  • OCT exposure led to increased MICs in one strain and decreased susceptibility to penicillin in another.

Conclusions:

  • Staphylococcal strains can develop resistance to antiseptics and cross-resistance to antibiotics.
  • Urgent need to implement regulated administration rules for antiseptics, similar to antibiotics.
  • Preserving the efficacy of both antiseptics and antibiotics is critical.
Keywords:
AntibioticAntisepticChlorhexidine digluconateOctenidine dihydrochlorideResistanceStaphylococcus spp.

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