Related Experiment Video

Updated: Jan 4, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

1.9K

The fungal mitochondrial Nad5 pan-genic intron landscape

Abdullah Zubaer1, Alvan Wai1, Georg Hausner1

  • 1Department of Microbiology, University of Manitoba, Winnipeg, Canada.

Mitochondrial DNA. Part A, DNA Mapping, Sequencing, and Analysis
|November 9, 2019
PubMed

Insights

Researchers mapped fungal mitochondrial nad5 gene introns, finding mostly group I introns. This intron landscape aids in predicting mobile introns in fungal mtDNA, useful for biotechnology applications.

Area of Science:

  • Mycology
  • Molecular Biology
  • Genomics

Background:

  • Fungal mitochondrial genomes contain introns that play roles in gene expression and evolution.
  • The nad5 gene is a crucial component of the mitochondrial respiratory chain in fungi.

Purpose of the Study:

  • To characterize the intron landscape of the fungal mitochondrial nad5 gene across diverse species.
  • To identify patterns in intron types, insertion sites, and their potential evolutionary significance.

Main Methods:

  • Bioinformatic analysis of nad5 gene sequences from 188 fungal species.
  • Classification of introns based on structural features and secondary RNA structures.
  • Analysis of intron insertion site characteristics, including reading frame bias.

Main Results:

  • A total of 265 introns were identified in 29 distinct insertion sites within fungal nad5 genes.
  • The vast majority (263) were group I introns, with only two group II introns found.
  • Intron insertion sites showed a bias towards phase 0 and appeared to be intron-type specific.

Conclusions:

  • The study provides a comprehensive map of intron distribution in the fungal mitochondrial nad5 gene.
  • Intron landscapes can serve as predictive tools for locating mobile introns in fungal mtDNA.
  • These mobile introns, containing ribozyme and potentially protein-coding elements, have significant biotechnological potential.
Keywords:
Core creepGroup I intronIntron insertion-sitesIntron phasing

More Related Videos

Methodology for Accurate Detection of Mitochondrial DNA Methylation12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

13.9K
Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe08:07

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe

Published on: May 2, 2025

843

Related Concept Videos

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
14.9K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
8.9K
Fungal Phylum Microsporidia01:28

Fungal Phylum Microsporidia

Microsporidia are a group of obligate intracellular fungi that were initially classified as protists but were later reclassified based on phylogenetic, molecular, and structural evidence linking them to the Chytridiomycota. These unicellular, non-motile organisms are highly specialized parasites that infect a wide range of animal hosts, including humans. They have evolved extensive genomic and metabolic reductions, making them highly dependent on their hosts for survival.Morphology and Genomic...
360
Export of Mitochondrial and Chloroplast Genes02:19

Export of Mitochondrial and Chloroplast Genes

A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
4.1K
Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
19.4K
Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
16.5K