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Related Experiment Videos

Glucan-binding factor in saliva.

M M Cowan1, K Parrish, R E Kessler

  • 1Health Sciences Center, University of Louisville, Kentucky 40292.

Infection and Immunity
|November 1, 1988
PubMed
Summary
This summary is machine-generated.

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Whole saliva contains a lipoteichoic acid component that solubilizes alcohol-insoluble D-glucans. This interaction may be crucial for oral streptococci adhesion to saliva-coated surfaces.

Area of Science:

  • Oral microbiology
  • Biochemistry
  • Polymer science

Background:

  • High-molecular-weight alpha-1,6-linked D-glucans are insoluble in alcohol.
  • Saliva plays a role in oral health, potentially interacting with bacterial products.

Purpose of the Study:

  • To identify the factor in whole saliva that renders D-glucans soluble in alcohol.
  • To investigate the nature and function of this glucan-binding factor.

Main Methods:

  • Ethanol precipitation of D-glucans.
  • Sephacryl S-200 gel filtration chromatography.
  • Passive hemagglutination assays.
  • Treatment with heat, proteases, and other agents.

Main Results:

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  • Whole saliva, but not parotid saliva, prevented D-glucan precipitation in 80% ethanol.
  • A glucan-binding factor was isolated and eluted with water from Sephacryl S-200.
  • The factor sensitized erythrocytes to agglutination with anti-poly(glycerolphosphate), suggesting lipoteichoic acid.
  • The factor was resistant to various treatments, including heat and proteases.
  • Sucrose and penicillin G released the factor from oral streptococci.

    • Conclusions:

    • Whole saliva contains a lipoteichoic acid component that complexes with D-glucans, increasing their solubility in hydrophobic solvents.
    • This complex formation may be significant for the adhesion of oral streptococci to saliva-coated surfaces.