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Related Experiment Video

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Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
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Hypervirulent clade 2, ribotype 019/sequence type 67 Clostridioides difficile strain from Japan.

Ryoichi Saito1,2, Yukino Usui1, Alafate Ayibieke1

  • 11Department of Molecular Microbiology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.

Gut Pathogens
|November 9, 2019
PubMed
Summary

This study reports the first genome of Clostridioides difficile ribotype 019/sequence type 67 in Japan. The findings enhance understanding of hypervirulent clade 2 lineages, including their genetic makeup and cytotoxicity.

Keywords:
Binary toxinCdt locusClostridioides difficileHypervirulent clade 2Pathogenicity locusRibotype 019Sequence type 67Toxin AToxin B

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Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Clostridioides difficile ribotype (RT) 019/sequence type (ST) 67 strains are a hypervirulent lineage related to RT027/ST1.
  • Limited data exists for hypervirulent clade 2 lineages in Japan.

Purpose of the Study:

  • To report the draft genome of a C. difficile strain B18-123, belonging to clade 2, RT019/ST67, for the first time in Japan.

Main Methods:

  • Genome sequencing and analysis
  • Pathogenicity locus comparison
  • Cytotoxicity assays
  • Antimicrobial susceptibility testing

Main Results:

  • The B18-123 strain's pathogenicity locus showed high homology to RT027/ST1 strain R20291.
  • The strain harbored 8-nucleotide substitutions in tcdC but lacked specific deletions found in R20291.
  • Cytotoxicity levels were similar to RT027/ST1 strains, and the strain was susceptible to metronidazole and vancomycin.

Conclusions:

  • This study provides insights into the characteristics of hypervirulent clade 2, specifically RT019/ST67 lineages in Japan.