Related Experiment Video
Updated: Jan 4, 2026
07:20
Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
Published on: January 31, 2025
1.1K
Endogenous leptin promotes autophagy in EBSS-induced PFCs
Deling Jiao1,2,3, Zhen Yang1,2,3, Lulu Wang1,2,4
1Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province, Kunming, People's Republic of China.
Animal Cells and Systems
|November 9, 2019
Summary
Leptin promotes autophagy in pig fibroblast cells by increasing autophagosome formation and LC3-II levels. This adipokine
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Leptin, an adipokine, is crucial in animal physiology.
- Its role in the autophagic response of pig fibroblast cells (PFCs) remains unclear.
Purpose of the Study:
- To investigate the relationship between leptin and autophagy in PFCs.
- To elucidate the molecular mechanisms underlying leptin's effect on autophagy.
Main Methods:
- Cultured leptin transgenic and wild-type (WT) PFCs.
- Treated cells with Earle's Balanced Salt Solution (EBSS) and Bafilomycin A1 (Baf A1).
- Assessed autophagy markers (LC3-II, p62), autophagosome formation (TEM), and gene expression (q-PCR).
Main Results:
- Leptin transgenic PFCs showed increased LC3-II and decreased p62 levels compared to WT PFCs.
- Enhanced autophagosome formation and LC3 puncta accumulation were observed in leptin transgenic PFCs.
- Significant differential gene expression was noted in autophagy signaling and ATG genes.
Conclusions:
- Leptin promotes autophagy in PFCs.
- Leptin's action may involve the downregulation of FOXO1 and LMNA genes.
- The precise pathway requires further investigation.
More Related Videos
11:39Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
Published on: July 21, 2017
32.1K
06:58Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles
Published on: October 18, 2024
1.1K
Related Concept Videos
01:27Autophagy
5.6K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.6K
01:36Delivery Pathways to the Lysosome
8.7K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
8.7K
01:15Receptor Downregulation in MVBs
2.7K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.7K
01:30Regulation of Food Intake
2.2K
Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...
2.2K
01:28The Early Endosome: Endocytosis of Transferrin
4.6K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
4.6K
01:18Autophagic Cell Death
4.3K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
4.3K