Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.8K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.8K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.1K
2.1K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.1K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.1K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.3K
5.3K
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

3.8K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
3.8K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dysregulated sphingolipid metabolismdrives pancreatic carcinogenesis through plasma membrane Kras enrichment.

Gut·2026
Same author

Identification of 2-Substituted-3-Hydroxy-2,3-Dihydroquinazolin-4(1H) Ones as Cell-Active Inhibitors of KDM4A Lysine Demethylase.

ChemMedChem·2026
Same author

A time-resolved atlas of histone modifications during mitotic entry.

Molecular cell·2026
Same author

Targeted degradation of Drosophila FOG homolog U-shaped mimics macrophage transdifferentiation in S2 cells.

The international journal of biochemistry & cell biology·2026
Same author

Spatially resolved mapping of histones reveals selective neuronal response in Rett syndrome.

The FEBS journal·2026
Same author

Dbf4-dependent kinase finetunes Ino80 function at chromosome replication origins.

Nature communications·2026
Same journal

Neurochondrin promotes U5 snRNP maturation by regulating AAR2 release from PRPF8.

Nucleic acids research·2026
Same journal

Elongationless start-stop elements are stress-resilient translation gates that are more repressive than uTranslons.

Nucleic acids research·2026
Same journal

Evolution of the ribosomal exit tunnel through the eyes of the nascent chain.

Nucleic acids research·2026
Same journal

Enhancing the performance and interpretability of epigenetic clocks.

Nucleic acids research·2026
Same journal

FABIAN-variant 2026: improved prediction of the effects of DNA variants on transcription factor binding.

Nucleic acids research·2026
Same journal

Structural and biochemical characterization of Grimontia hollisae thermostable direct hemolysin with DNA reveals first Vibrio hemolysin with nuclease activity.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: Jan 4, 2026

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
11:50

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Published on: June 23, 2022

2.5K

Distinct CoREST complexes act in a cell-type-specific manner.

Igor Mačinković1, Ina Theofel2, Tim Hundertmark2

  • 1Institute of Molecular Biology and Tumor Research, Biomedical Research Center, Philipps-University, Hans-Meerwein-Strasse 2, 35043, Marburg, Germany.

Nucleic Acids Research
|November 9, 2019
PubMed
Summary
This summary is machine-generated.

Three distinct CoREST complexes regulate gene expression in a cell-specific manner. These complexes, including dLSD1/CoREST, LINT, and dG9a/CoREST, are crucial for maintaining distinct cell lineages and developmental programs.

More Related Videos

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

6.2K
Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis
12:48

Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis

Published on: March 25, 2014

15.3K

Related Experiment Videos

Last Updated: Jan 4, 2026

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
11:50

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Published on: June 23, 2022

2.5K
In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

6.2K
Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis
12:48

Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis

Published on: March 25, 2014

15.3K

Area of Science:

  • Epigenetics and Gene Regulation
  • Developmental Biology
  • Proteomics

Background:

  • Co-Repressor of Repressor Element 1-Silencing Transcription factor (CoREST) is a key component of chromatin-modifying complexes.
  • Previous studies identified CoREST in transcriptionally repressive complexes, but a comprehensive interactome analysis was lacking.

Purpose of the Study:

  • To define the interactomes of two Drosophila CoREST isoforms (dCoREST-L and dCoREST-M).
  • To investigate the functional roles of distinct CoREST-containing complexes in different cell types.

Main Methods:

  • Proteomic approaches to identify CoREST interactomes in Drosophila.
  • Genome-wide chromatin association studies.
  • Transcriptome analyses in S2 cells and testes.

Main Results:

  • Identified three distinct histone deacetylase complexes: dLSD1/dCoREST, LINT, and dG9a/dCoREST.
  • dLSD1/dCoREST complex exclusively uses dCoREST-L, while LINT and dG9a/dCoREST incorporate both isoforms.
  • These complexes associate with chromatin at promoters and exhibit lineage-restricted functions.
  • In S2 cells, LINT represses germline genes; in testes, dLSD1/dCoREST prevents inappropriate transcription and is vital for spermatogenesis.

Conclusions:

  • Uncovered three distinct dCoREST complexes with lineage-restricted roles in gene repression.
  • These complexes are essential for maintaining cell-type-specific gene expression programs during development.