Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of short and long-term cadmium exposure on behaviour and cardiac function in Drosophila.

Environmental pollution (Barking, Essex : 1987)·2024
Same author

Description and Cross-Sectional Analyses of 25,880 Adults and Children in the UK National Registry of Rare Kidney Diseases Cohort.

Kidney international reports·2024
Same author

Adiponectin Reduces Glomerular Endothelial Glycocalyx Disruption and Restores Glomerular Barrier Function in a Mouse Model of Type 2 Diabetes.

Diabetes·2024
Same author

Effects of rare kidney diseases on kidney failure: a longitudinal analysis of the UK National Registry of Rare Kidney Diseases (RaDaR) cohort.

Lancet (London, England)·2024
Same author

A small molecule chaperone rescues keratin-8 mediated trafficking of misfolded podocin to correct genetic Nephrotic Syndrome.

Kidney international·2023
Same author

Adeno-associated virus gene therapy prevents progression of kidney disease in genetic models of nephrotic syndrome.

Science translational medicine·2023
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jan 4, 2026

Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies
08:52

Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies

Published on: May 26, 2023

2.9K

Modeling Podocyte Biology Using Drosophila Nephrocytes.

Paul S Hartley1, Richard J Coward2

  • 1Department of Life and Environmental Science, Bournemouth University, Poole, Dorset, UK. phartley@bournemouth.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2019
PubMed
Summary
This summary is machine-generated.

Drosophila nephrocytes offer a rapid model for studying human kidney podocyte function and slit diaphragm formation. These methods enable research into the genetic basis of kidney diseases like albuminuria and renal failure.

Keywords:
DrosophilaKidneyNephrocyteNephropathyPodocyteSlit diaphragm

More Related Videos

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions
08:06

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions

Published on: July 2, 2020

5.0K
Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells
10:23

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells

Published on: November 4, 2022

3.5K

Related Experiment Videos

Last Updated: Jan 4, 2026

Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies
08:52

Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies

Published on: May 26, 2023

2.9K
Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions
08:06

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions

Published on: July 2, 2020

5.0K
Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells
10:23

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells

Published on: November 4, 2022

3.5K

Area of Science:

  • Nephrology
  • Developmental Biology
  • Genetics

Background:

  • Vertebrate podocytes are crucial glomerular cells regulating kidney filtration via slit diaphragms.
  • Disrupted podocyte function leads to human kidney diseases, including albuminuria and renal failure.
  • Drosophila nephrocytes share genetic and functional similarities with human podocytes.

Purpose of the Study:

  • To present methods for modulating and studying Drosophila nephrocyte function.
  • To investigate Drosophila nephrocyte diaphragm formation as a model for human podocyte biology.
  • To facilitate research into the genetic and metabolic basis of human kidney diseases.

Main Methods:

  • Utilizing Drosophila as a model organism for nephrocyte research.
  • Employing genetic and transgenic techniques to modulate nephrocyte function.
  • Developing and applying methods to study slit diaphragm formation in Drosophila.

Main Results:

  • Drosophila nephrocytes provide a rapid and effective system for studying podocyte biology.
  • Established methods allow for detailed analysis of nephrocyte function and diaphragm assembly.
  • The study facilitates comparative analysis between Drosophila and human kidney disease models.

Conclusions:

  • Drosophila nephrocytes are a valuable tool for understanding human podocyte biology and kidney disease.
  • The presented methods accelerate research into the genetic underpinnings of renal filtration and disease.
  • This model system aids in the development of therapeutic strategies for kidney disorders.