Assessment of Urinary MicroRNAs by Quantitative Polymerase Chain Reaction in Diabetic Nephropathy Patients
Lucy Jade Newbury1, Alexa Wonnacott1, Kate Simpson1
1Wales Kidney Research Unit, Heath Park Campus, Cardiff, UK.
Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2019
Abstract:
Urinary microRNAs show promise as noninvasive biomarkers in renal disease. Here, we describe a detailed protocol for the column-based extraction and quantification of miRNAs by reverse-transcription quantitative polymerase chain reaction (RT-qPCR) from urine samples.


