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Reintroducing mothur: 10 Years Later.

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  • 1Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA pschloss@umich.edu.

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Summary
This summary is machine-generated.

The mothur software package provides robust amplicon sequence analysis for microbial ecology. Its decade-long development highlights the importance of iterative scientific progress, balancing current limitations with future advancements in sequencing technology.

Keywords:
16S rRNA geneampliconbioinformaticsdata analysismicrobial ecologymicrobiome

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Area of Science:

  • Microbial Ecology
  • Bioinformatics
  • Computational Biology

Background:

  • The mothur software package was developed over a decade ago to address the need for robust amplicon sequence data analysis.
  • It has played a significant role in advancing microbial ecology research during the sequencing revolution.

Purpose of the Study:

  • To create a comprehensive software package for analyzing amplicon sequence data.
  • To provide the microbial ecology community with robust and accessible analysis tools.

Main Methods:

  • Development of a comprehensive software package (mothur) for sequence analysis.
  • Iterative improvement of tools based on scientific advancements and community needs.

Main Results:

  • mothur has become a widely used tool in microbial ecology.
  • Its development provided insights into the nature of scientific progress, emphasizing the balance between current limitations and technological evolution.

Conclusions:

  • Scientific progress involves addressing immediate problems while acknowledging evolving technologies and ideas.
  • The mothur package demonstrates the value of adaptable, data-driven development in scientific software.