The Histone Demethylase NO66 Induces Glioma Cell Proliferation
Qing Wang1, Pei Y Liu2, Tao Liu2
1Department of Neurosurgery, the Second Affiliated Hospital of Soochow University, Suzhou, P.R. China.
Anticancer Research
|November 10, 2019
Summary
High NO66 levels in glioma and glioblastoma predict poor prognosis. NO66 is essential for epidermal growth factor receptor (EGFR) expression and glioblastoma cell growth.
Area of Science:
- Oncology
- Molecular Biology
- Epigenetics
Background:
- The histone demethylase NO66 influences gene and protein expression.
- Epidermal growth factor receptor (EGFR) is a critical oncogenic factor in glioblastoma development.
Purpose of the Study:
- To investigate the role of NO66 in glioblastoma.
- To determine the correlation between NO66 expression and patient prognosis.
Main Methods:
- Kaplan-Meier survival analysis of NO66 expression in 263 glioma and 510 glioblastoma tissues.
- Assessment of EGFR expression, cell proliferation, and cell cycle via immunoblot analysis and assays following NO66 knockdown in glioblastoma cells.
Main Results:
- High NO66 expression correlated with advanced stage and poor prognosis in glioma tissues.
- Elevated NO66 levels predicted poor prognosis in glioblastoma tissues.
- NO66 knockdown led to reduced EGFR expression and cell proliferation in glioblastoma cells.
Conclusions:
- High NO66 expression serves as a predictive marker for poor prognosis in glioma and glioblastoma.
- NO66 is indispensable for maintaining EGFR expression and driving glioblastoma cell proliferation.
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