Related Experiment Video

Updated: Jan 4, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.6K

Role of Phosphodiesterase2A in Proliferation and Migration of Human Osteosarcoma Cells

Taku Murata1, Kasumi Shimizu2, Kazuto Kurohara2

  • 1Department of Oral and Maxillofacial Surgery, Department of Clinical Sciences, Medical Life Science, Mie University, Graduate School of Medicine, Mie, Japan muratat@clin.medic.mie-u.ac.jp.

Anticancer Research
|November 10, 2019
PubMed
Abstract

Insights

New research reveals that phosphodiesterase 2 (PDE2) impacts osteosarcoma cell behavior. PDE2-cAMP signaling inhibits cell proliferation, while PDE2-cGMP signaling promotes cell migration in oral osteosarcoma cells.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Signaling

Background:

  • Osteosarcoma has a poor prognosis, necessitating novel therapeutic strategies.
  • Phosphodiesterase 2 (PDE2) regulates intracellular cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP).
  • This study investigates PDE2's role in human oral osteosarcoma HOSM-1 cells.

Purpose of the Study:

  • To determine the expression of PDE2 in HOSM-1 cells.
  • To elucidate the effects of PDE2 inhibition on osteosarcoma cell proliferation and migration.
  • To understand the signaling pathways regulated by PDE2 in osteosarcoma.

Main Methods:

  • PDE2 expression was quantified using a cAMP-PDE assay and real-time PCR.
  • The impact of PDE2 inhibitors (EHNA, 8-bromo-cAMP, 8-bromo-cGMP) on cell proliferation and migration was evaluated.
  • In vitro assays were performed on human oral osteosarcoma HOSM-1 cells.

Main Results:

  • PDE2 activity and PDE2A mRNA were detected in HOSM-1 cells.
  • Inhibition of PDE2 by EHNA and 8-bromo-cAMP reduced cell proliferation.
  • Cell migration was enhanced by EHNA and 8-bromo-cGMP, but inhibited by 8-bromo-cAMP.

Conclusions:

  • PDE2-cAMP signaling negatively regulates cell proliferation in HOSM-1 cells.
  • PDE2-cGMP signaling positively regulates cell migration in HOSM-1 cells.
  • PDE2 plays distinct roles in regulating osteosarcoma cell proliferation and migration.
Keywords:
Phosphodiesterasemigrationosteosarcomaphosphodiesterase2Aproliferation

More Related Videos

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate07:31

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

4.1K
A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

10.6K

Related Concept Videos

Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
3.1K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.6K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
14.8K