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Updated: Jan 4, 2026

HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice
Published on: July 9, 2016
Unstructured loop is essential for the activation of mGluR2
Deo R Singh1, Ashish K Mishra2, Pankaj Pandey3
1Department of Biochemistry, Weill Cornell Medical College, New York City, NY, USA; Department of Cell and Molecular Physiology, Stritch School of Medicine, Maywood, IL, USA.
Abstract:
Metabotropic Glutamate Receptors (mGluRs) are Class C G-protein coupled receptors (GPCRs) that are expressed throughout the central nervous system and are involved in several neurological and psychiatric disorders. Although, many studies focused on Glutamate induced activation of mGluR2, however, the role of unstructured loop (or "BC loop") in activation of metabotropic Glutamate receptors is currently unknown. Here, using Förster Resonance Energy Transfer (FRET) based assay in live cells we show that unstructured loop is required for Glutamate induced conformation and hence the activation of the receptor.
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