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Bioanalysis of (E)-5-(2-bromovinyl)-2'-deoxyuridine
H J Reeuwijk1, H Lingeman, U R Tjaden
1Center for Bio-Pharmaceutical Sciences, Leiden University, The Netherlands.
Journal of Chromatography
|June 24, 1988
Summary
(E)-5-(2-Bromovinyl)-2'-deoxyuridine, an antiviral, can be quantified in biological samples. This method enables pharmacokinetic studies of this potential anticancer drug.
Area of Science:
- Pharmacology and Toxicology
- Analytical Chemistry
- Biochemistry
Background:
- Antiviral drugs are crucial in cancer therapy, often modulating existing treatments.
- (E)-5-(2-Bromovinyl)-2'-deoxyuridine is an experimental antiviral agent studied for its potential to enhance anticancer effects of fluoropyrimidines.
- Accurate bioanalysis is essential for understanding drug efficacy and patient response.
Purpose of the Study:
- To develop and validate a bioanalytical method for quantifying (E)-5-(2-Bromovinyl)-2'-deoxyuridine in biological matrices.
- To establish a method suitable for pharmacokinetic studies of the drug.
- To assess the drug's behavior in plasma and urine.
Main Methods:
- Sample preparation involved protein precipitation for plasma and liquid-liquid extraction for urine.
- Analysis was performed using reversed-phase chromatography.
- Quantification was achieved via absorbance detection at 307 nm.
Main Results:
- The method achieved a minimum detectable concentration of approximately 6 ng/ml in both plasma and urine.
- Recovery rates were 75% for plasma and 92% for urine.
- High protein binding (97%) was observed for the analyte.
Conclusions:
- A reliable bioanalytical method for (E)-5-(2-Bromovinyl)-2'-deoxyuridine has been established.
- The method is suitable for supporting pharmacokinetic investigations.
- The drug's properties, including recovery and protein binding, are characterized.