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Cell-mediated immune response associated with Chlamydia pneumoniae infection in atherosclerotic patients

Loubna El Yazouli1, Fouad Seghrouchni2, Hicham Hejaji3

  • 1Institut Pasteur du Maroc, Chlamydiae and Mycoplasmas Laboratory, Casablanca, 20360, Morocco; Laboratory of Human Pathologies Biology, Genomic Center of Human Pathologies, Faculty of Sciences, Mohammed V University of Rabat, Morocco.

Microbial Pathogenesis
|November 11, 2019
PubMed

Insights

Chlamydia pneumoniae infection increases CD4, CD8, and CD14 expression in atherosclerosis patients and healthy individuals. This suggests C. pneumoniae may drive immune responses contributing to atherosclerosis development.

Area of Science:

  • Immunology
  • Infectious Diseases
  • Cardiovascular Research

Background:

  • Chlamydia pneumoniae is an intracellular bacterium linked to atherosclerosis.
  • It activates cell-mediated immune responses, suggesting a role in cardiovascular disease pathogenesis.

Purpose of the Study:

  • To investigate the cell-mediated immune response to C. pneumoniae in atherosclerosis patients.
  • To evaluate the expression of CD14, CD8, and CD4 in response to C. pneumoniae infection.

Main Methods:

  • Analyzed 27 atherosclerosis patients and 32 controls.
  • Detected C. pneumoniae DNA in peripheral blood mononuclear cells (PBMCs) using nested PCR.
  • Measured CD4, CD8, and CD14 expression via flow cytometry.

Main Results:

  • Increased mean fluorescence intensity (MFI) of CD4, CD8, and CD14 was observed in C. pneumoniae DNA-positive subjects (patients and controls).
  • Statistically significant differences (p < 0.05) were found in marker expression between C. pneumoniae DNA-positive and negative groups.
  • C. pneumoniae DNA-positive atherosclerosis patients showed significantly higher CD4, CD8, and CD14 expression compared to C. pneumoniae DNA-positive healthy controls.

Conclusions:

  • C. pneumoniae infection significantly alters cell-mediated immune responses.
  • Elevated CD4, CD8, and CD14 expression suggests a role for C. pneumoniae in atherosclerosis-related inflammation and thrombosis.
  • Further research is warranted to understand C. pneumoniae's impact on immune mechanisms in atherosclerosis.
Abstract

Keywords:
Cell immune responseChlamydia pneumoniaeFlow cytometry

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